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GDF11 Antibody (N-term)

Catalog # Availability Size / Price Inquiry
APR12009G 100 μl / $495

GDF11 Antibody (N-term)

Brand

Leading Biology

Catalog Number

APR12009G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality GDF11 antibody (N-term).

Molecular Weight

45091 Da

Cellular Localization

Antigen Cellular Localization: Secreted.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

32-61 aa

Target

This GDF11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-61 amino acids from the N-terminal region of human GDF11.

Isotype

Rabbit Ig

Symbol

BMP11

GeneID

UniProt ID

Function

Secreted signal that acts globally to specify positional identity along the anterior/posterior axis during development. Play critical roles in patterning both mesodermal and neural tissues and in establishing the skeletal pattern.

Summary

GDF11 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice and Xenopus suggest that this protein is involved in mesodermal formation and neurogenesis during embryonic development.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, E

Dilution

WB~~1:1000 IHC-P~~1:10~50

Synonyms

Growth/differentiation factor 11, GDF-11, Bone morphogenetic protein 11, BMP-11, GDF11, BMP11

Images

All lanes : Anti-hGDF11-R47 at 1:2000 dilution Lane 1: human heart lysate Lane 2: K562 whole cell lysate Lane 3: mouse kidney lysate Lane 4: 293 whole cell lysate Lane 5: Hela whole cell lysate Lane 5: mouse brain lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of GDF11 (arrow) using rabbit polyclonal GDF11 Antibody (R47) (Cat. APR12009G).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GDF11 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with GDF11 antibody (N-term) (Cat. APR12009G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specification

Quantity

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