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DMRTA2 Antibody (C-term)

APR15751G Product Datasheet Protocol

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APR15751G		100 μl / $495

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Leading Biology Inc.

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DMRTA2 Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR15751G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality DMRTA2 antibody (C-term).
Molecular Weight	53356 Da
Cellular Localization	Antigen Cellular Localization: Nucleus {ECO:0000255\|PROSITE- ProRule:PRU00070}
Host	Rabbit
Species Reactivity	Human, Mouse, Zebrafish
Immunogen	487-515 aa
Target	This DMRTA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487-515 amino acids from the C-terminal region of human DMRTA2.
Isotype	Rabbit Ig
Symbol	DMRT5
GeneID	63950
UniProt ID	Q96SC8
Function	May be involved in sexual development.
Summary	DMRTA2 may be involved in sexual development.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IF, E
Dilution	WB~~1:1000 IF~~1:10~50
Synonyms	Doublesex- and mab-3-related transcription factor A2, Doublesex- and mab-3-related transcription factor 5, DMRTA2, DMRT5
Images	DMRTA2 Antibody (C-term) (Cat. APR15751G) western blot analysis in PC-3 cell line, mouse testis and zebra fish brain tissue lysates (35ug/lane).This demonstrates the DMRTA2 antibody detected the DMRTA2 protein (arrow). Fluorescent confocal image of U251 cell stained with DMRTA2 Antibody (C-term)( APR15751G).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DMRTA2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min). DMRTA2 immunoreactivity is localized to Microtubules significantly.
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