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PIP5KL1 Antibody (N-term)

AMM07198G Product Datasheet Protocol

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AMM07198G		100 μl / $495

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Leading Biology Inc.

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PIP5KL1 Antibody (N-term)

Brand	Leading Biology	Catalog Number	AMM07198G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PIP5KL1 antibody (N-term).
Molecular Weight	44572 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm. Membrane. Note=Localized to large cytoplasmic vesicular structures.
Host	Rabbit
Species Reactivity	Human
Immunogen	57-86 aa
Target	This PIP5KL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-86 amino acids from the N-terminal region of human PIP5KL1.
Isotype	Rabbit Ig
GeneID	138429
UniProt ID	Q5T9C9
Function	May act as a scaffold to localize and regulate type I PI(4)P 5-kinases to specific compartments within the cell, where they generate PI(4,5)P2 for actin nucleation, signaling and scaffold protein recruitment and conversion to PI(3,4,5)P3.
Summary	PIP5KL1 is a phosphoinositide kinase-like protein thatlacks intrinsic lipid kinase activity but associates with type IPIPKs (see PIP5K1A; MIM 603275) and may play a role in localizationof PIPK activity (Chang et al., 2004 [PubMed 14701839]).[suppliedby OMIM].
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, IF, E
Dilution	WB~~1:1000 IHC-P~~1:50~100 IF~~1:10~50
Synonyms	Phosphatidylinositol 4-phosphate 5-kinase-like protein 1, PI(4)P 5-kinase-like protein 1, PtdIns(4)P-5-kinase-like protein 1, PIP5KL1
Images	Western blot analysis of lysates from 293,K562,PC-3 cell line (from left to right),using PIP5KL1 Antibody (N-term)(Cat. AMM07198G).AMM07198G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane. PIP5KL1 Antibody (N-term) (Cat. AMM07198G)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PIP5KL1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. Confocal immunofluorescent analysis of PIP5KL1 Antibody (N-term)( AMM07198G) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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