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CTSA Antibody (N-term)

APR07094G Product Datasheet Protocol

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APR07094G		100 μl / $495

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Leading Biology Inc.

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CTSA Antibody (N-term)

Brand	Leading Biology	Catalog Number	APR07094G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CTSA antibody (N-term).
Molecular Weight	54466 Da
Cellular Localization	Antigen Cellular Localization: Lysosome.
Host	Rabbit
Species Reactivity	Human, Mouse
Immunogen	18-45 aa
Target	This CTSA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-45 amino acids from the N-terminal region of human CTSA.
Isotype	Rabbit Ig
Symbol	PPGB
GeneID	5476
UniProt ID	P10619
Function	Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins.
Summary	CTSA encodes a glycoprotein which associates withlysosomal enzymes beta-galactosidase and neuraminidase to form acomplex of high molecular weight multimers. The formation of thiscomplex provides a protective role for stability and activity.Deficiencies in this gene are linked to multiple forms ofgalactosialidosis.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, E
Dilution	IHC-P~~1:50~100 WB~~1:1000
Synonyms	Lysosomal protective protein, Carboxypeptidase C, Carboxypeptidase L, Cathepsin A, Protective protein cathepsin A, PPCA, Protective protein for beta-galactosidase, Lysosomal protective protein 32 kDa chain, Lysosomal protective protein 20 kDa chain, CTSA, PPGB
Images	Immunohistochemical analysis of paraffin-embedded H. kidney section using CTSA Antibody (N-term)( APR07094G). APR07094G was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining. Western blot analysis of lysates from A431, Hela, HepG2, HT-1080 cell line and human placenta, kidney tissue lysate (from left to right), using CTSA Antibody (N-term)( APR07094G). APR07094G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane. CTSA Antibody (N-term) (Cat. APR07094G) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CTSA antibody detected the CTSA protein (arrow).
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