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CHRM2 Antibody

AMM05766G Product Datasheet Protocol

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AMM05766G		100 μl / $495

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Leading Biology Inc.

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CHRM2 Antibody

Brand	Leading Biology	Catalog Number	AMM05766G
Product Type	Monoclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CHRM2 antibody.
Molecular Weight	51715 Da
Cellular Localization	Antigen Cellular Localization: Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Note=Phosphorylation in response to agonist binding promotes receptor internalization.
Host	Mouse
Species Reactivity	Human, Mouse
Immunogen	Recombinant Protein aa
Target	This antibody is generated from a mouse immunized with a recombinant protein.
Clone	1424CT461.78.60
Isotype	IgG1,κ
GeneID	1129
UniProt ID	P08172
Function	The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3); this then triggers calcium ion release into the cytosol.
Summary	The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.
Form	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	IF, WB, IHC-P, FC, E
Dilution	FC~~1:25 IHC-P~~1:25 IF~~1:25 WB~~1:500
Synonyms	Muscarinic acetylcholine receptor M2, CHRM2
Images	Overlay histogram showing SH-SY5Y cells stained with AMM05766G (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Alexa Fluor? 488 goat anti-mouse lgG (166821) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. Immunohistochemical analysis of paraffin-embedded H. brain section using CHRM2( AMM05766G ). AMM05766G was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining. Fluorescent image of SH-SY5Y cells stained with CHRM2 Antibody ( AMM05766G ). AMM05766G was diluted at 1:25 dilution. An Alexa Fluor? 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
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