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MAP3K13 (LZK) Antibody (C-term)

APR17284G Product Datasheet Protocol

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APR17284G		100 μl / $495

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Leading Biology Inc.

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MAP3K13 (LZK) Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR17284G
Product Type	Monoclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality MAP3K13 (LZK) antibody (C-term).
Molecular Weight	108296 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm. Membrane; Peripheral membrane protein
Host	Mouse
Species Reactivity	Human
Immunogen	52-305 aa
Target	Purified His-tagged MAP3K13 protein fragment was used to produced this monoclonal antibody.
Clone	810CT26.6.1.1
Isotype	IgM
GeneID	9175
UniProt ID	O43283
Function	Activates the JUN N-terminal pathway through activation of the MAP kinase kinase MAP2K7. Acts synergistically with PRDX3 to regulate the activation of NF-kappa-B in the cytosol. This activation is kinase-dependent and involves activating the IKK complex, the IKBKB-containing complex that phosphorylates inhibitors of NF-kappa-B.
Summary	Activates the JUN N-terminal pathway through activation of the MAP kinase kinase MAP2K7. Acts synergistically with PRDX3 to regulate the activation of NF-kappa-B in the cytosol. This activation is kinase-dependent and involves activating the IKK complex, the IKBKB-containing complex that phosphorylates inhibitors of NF-kappa-B.
Form	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Euglobin precipitation followed by dialysis against PBS.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IF, E
Dilution	IF~~1:100 WB~~1:1000
Synonyms	Mitogen-activated protein kinase kinase kinase 13, Leucine zipper-bearing kinase, Mixed lineage kinase, MLK, MAP3K13 (HGNC:6852)
Images	Fluorescent image of NCI-H460 cells stained with MAP3K13 (LZK) Antibody (N-term)( AM2234B). AM2234B was diluted at 1:100 dilution. An Alexa Fluor? 488-conjugated goat anti-mouse lgM at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Fluorescent image of NCI-H460 cells stained with MAP3K13 (LZK) Antibody (N-term)( AM2234B). AM2234B was diluted at 1:100 dilution. An Alexa Fluor? 488-conjugated goat anti-mouse lgM at 1:400 dilution was used as the secondary antibody (green). Fluorescent image of NCI-H460 cells stained with MAP3K13 (LZK) Antibody (N-term)( AM2234B). AM2234B was diluted at 1:100 dilution. An Alexa Fluor? 488-conjugated goat anti-mouse lgM at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red).
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