> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > CHAT Antibody (N-term)Brand |
Leading Biology | Catalog Number |
AMM05746G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality CHAT antibody (N-term).
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Molecular Weight |
82536 Da
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Host |
Rabbit
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Species Reactivity |
Human, Mouse
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Immunogen |
98-128 aa
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Target |
This CHAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-128 amino acids from the N-terminal region of human CHAT.
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Isotype |
Rabbit Ig
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GeneID |
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UniProt ID |
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Function |
Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
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Summary |
CHAT is an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer disease.
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Form |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IF, E
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Dilution |
WB~~1:1000
IF~~1:10~50
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Synonyms |
Choline O-acetyltransferase, CHOACTase, ChAT, Choline acetylase, CHAT
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Images |
Western blot analysis of CHAT Antibody (N-term) (Cat. AMM05746G) in NCI-H460 cell line lysates (35ug/lane).CHAT (arrow) was detected using the purified Pab.
Western blot analysis of CHAT Antibody (N-term) (Cat. AMM05746G) in mouse liver tissue lysates (35ug/lane). CHAT (arrow) was detected using the purified Pab.
Confocal immunofluorescent analysis of CHAT Antibody (N-term) (Cat. AMM05746G) with NCI-H460 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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