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DIO3 Antibody (C-term)

APR07584G Product Datasheet Protocol

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APR07584G		100 μl / $495

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Leading Biology Inc.

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DIO3 Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR07584G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality DIO3 antibody (C-term).
Molecular Weight	33947 Da
Cellular Localization	Antigen Cellular Localization: Cell membrane; Single-pass type II membrane protein. Endosome membrane; Single-pass type II membrane protein
Host	Rabbit
Species Reactivity	Human
Immunogen	250-278 aa
Target	This DIO3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-278 amino acids from the C-terminal region of human DIO3.
Isotype	Rabbit Ig
Symbol	ITDI3, TXDI3
GeneID	1735
UniProt ID	P55073
Function	Responsible for the deiodination of T4 (3,5,3',5'- tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development.
Summary	DIO3 belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. This enzyme is highly expressed in the pregnant uterus, placenta, fetal and neonatal tissues, suggesting that it plays an essential role in the regulation of thyroid hormone inactivation during embryological development. This protein contains a selenocysteine (Sec) residue, which is essential for efficient enzyme activity. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, E
Dilution	WB~~1:1000 IHC-P~~1:50~100
Synonyms	Type III iodothyronine deiodinase, 5DIII, DIOIII, Type 3 DI, Type-III 5'-deiodinase, DIO3, ITDI3, TXDI3
Images	Western blot analysis of DIO3 Antibody (C-term) (Cat. APR07584G) in HepG2 cell line lysates (35ug/lane). DIO3 (arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with DIO3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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