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GCLM Antibody (C-term)

Catalog # Availability Size / Price Inquiry
AMM04739G 100 μl / $495

GCLM Antibody (C-term)

Brand

Leading Biology

Catalog Number

AMM04739G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality GCLM antibody (C-term).

Molecular Weight

30727 Da

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

246-274 aa

Target

This GCLM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 246-274 amino acids from the C-terminal region of human GCLM.

Isotype

Rabbit Ig

Symbol

GLCLR

GeneID

UniProt ID

Summary

GCLM known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, IF, E

Dilution

WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 IF~~1:10~50

Synonyms

Glutamate--cysteine ligase regulatory subunit, GCS light chain, Gamma-ECS regulatory subunit, Gamma-glutamylcysteine synthetase regulatory subunit, Glutamate--cysteine ligase modifier subunit, GCLM, GLCLR

Images

Anti-GCLM Antibody (C-term) at 1:8000 dilution + Hela whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of GCLM Antibody (C-term) (Cat. AMM04739G) in 293 cell line and mouse kidney tissue lysates (35ug/lane). GCLM (arrow) was detected using the purified Pab.

GCLM Antibody (C-term)(Cat. AMM04739G) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GCLM Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Specification

Quantity

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