> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > KIF11 Antibody (N-term)Brand |
Leading Biology | Catalog Number |
APR16994G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality KIF11 antibody (N-term).
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Molecular Weight |
119159 Da
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Cellular Localization |
Antigen Cellular Localization:
Cytoplasm. Cytoplasm, cytoskeleton, spindle pole
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Host |
Rabbit
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Species Reactivity |
Human, Hamster
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Immunogen |
103-130 aa
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Target |
This KIF11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-130 amino acids from the N-terminal region of human KIF11.
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Isotype |
Rabbit Ig
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Symbol |
EG5, KNSL1, TRIP5
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GeneID |
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UniProt ID |
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Function |
Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.
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Summary |
KIF11 belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis.
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Form |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IHC-P, FC, E
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Dilution |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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Synonyms |
Kinesin-like protein KIF11, Kinesin-like protein 1, Kinesin-like spindle protein HKSP, Kinesin-related motor protein Eg5, Thyroid receptor-interacting protein 5, TR-interacting protein 5, TRIP-5, KIF11, EG5, KNSL1, TRIP5
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Images |
Anti-KIF11 Antibody (N-term) at 1:1000 dilution + A431 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 119 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of KIF11 Antibody (N-term) (Cat. APR16994G) in CHO cell line lysates (35ug/lane). KIF11 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with KIF11 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Specification |
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Quantity |
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Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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