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RPGRIP1 Antibody (Center)

Catalog # Availability Size / Price Inquiry
APR13147G 100 μl / $495

RPGRIP1 Antibody (Center)

Brand

Leading Biology

Catalog Number

APR13147G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality RPGRIP1 antibody (Center).

Molecular Weight

146682 Da

Cellular Localization

Antigen Cellular Localization: Cell projection, cilium. Note=Situated between the axonemal microtubules and the plasma membrane {ECO:0000250|UniProtKB:Q9EPQ2}

Host

Rabbit

Species Reactivity

Human

Immunogen

530-557 aa

Target

This RPGRIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 530-557 amino acids from the Central region of human RPGRIP1.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

May function as scaffolding protein. Required for normal location of RPGR at the connecting cilium of photoreceptor cells. Required for normal disk morphogenesis and disk organization in the outer segment of photoreceptor cells and for survival of photoreceptor cells.

Summary

RPGRIP1 is a photoreceptor protein that interacts with retinitis pigmentosa GTPase regulator protein and is a key component of cone and rod photoreceptor cells.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, E

Dilution

WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50

Synonyms

X-linked retinitis pigmentosa GTPase regulator-interacting protein 1, RPGR-interacting protein 1, RPGRIP1

Images

Western blot analysis of RPGRIP1 Antibody (Center) (Cat. APR13147G) in HL-60, K562, MDA-MB435, Jurkat cell line lysates (35ug/lane). RPGRIP1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with RPGRIP1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

RPGRIP1 Antibody (Center) (Cat. APR13147G) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specification

Quantity

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