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NIP7 Antibody (C-term)

Catalog # Availability Size / Price Inquiry
APR17569G 100 μl / $495

NIP7 Antibody (C-term)

Brand

Leading Biology

Catalog Number

APR17569G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality NIP7 antibody (C-term).

Molecular Weight

20463 Da

Cellular Localization

Antigen Cellular Localization: Nucleus, nucleolus

Host

Rabbit

Species Reactivity

Human

Immunogen

150-177 aa

Target

This NIP7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150-177 amino acids from the C-terminal region of human NIP7.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

Required for proper 34S pre-rRNA processing and 60S ribosome subunit assembly.

Summary

Required for proper 27S pre-rRNA processing and 60S ribosome subunit assembly.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, FC, E

Dilution

WB~~1:1000 FC~~1:10~50

Synonyms

60S ribosome subunit biogenesis protein NIP7 homolog, KD93, NIP7

Images

Anti-NIP7 Antibody (C-term) at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 20 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

NIP7 Antibody (C-term) (Cat. APR17569G) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of NIP7 (arrow) using rabbit polyclonal NIP7 Antibody (C-term) (Cat. APR17569G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NIP7 gene.

Specification

Quantity

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