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CYP4F3 Antibody (N-term)

Catalog # Availability Size / Price Inquiry
APG02897G 100 μl / $495

CYP4F3 Antibody (N-term)

Brand

Leading Biology

Catalog Number

APG02897G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CYP4F3 antibody (N-term).

Molecular Weight

59847 Da

Cellular Localization

Antigen Cellular Localization: Endoplasmic reticulum membrane; Single-pass membrane protein Microsome membrane; Single-pass membrane protein

Host

Rabbit

Species Reactivity

Human

Immunogen

77-109 aa

Target

This CYP4F3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-109 amino acids from the N-terminal region of human CYP4F3.

Isotype

Rabbit Ig

Symbol

LTB4H

GeneID

UniProt ID

Function

Isoform CYP4F3A: Catalyzes the omega-hydroxylation of leukotriene-B(4), a potent chemoattractant for polymorphonuclear leukocytes, it has low activity for arachidonic acid.

Summary

CYP4F3 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. The enzyme starts the process of inactivating and degrading leukotriene B4, a potent mediator of inflammation.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, FC, E

Dilution

WB~~1:1000 FC~~1:10~50

Synonyms

Docosahexaenoic acid omega-hydroxylase CYP4F3, 20-hydroxyeicosatetraenoic acid synthase, 20-HETE synthase, 11413-, CYPIVF3, Cytochrome P450 4F3, Cytochrome P450-LTB-omega, Leukotriene-B(4) 20-monooxygenase 2, Leukotriene-B(4) omega-hydroxylase 2, CYP4F3, LTB4H

Images

Western blot analysis of CYP4F3 Antibody (N-term) (Cat. APG02897G) in K562 cell line lysates (35ug/lane). CYP4F3 (arrow) was detected using the purified Pab.

CYP4F3 Antibody (N-term) (Cat. APG02897G) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specification

Quantity

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