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PPP2R2B Antibody (Center)

Catalog # Availability Size / Price Inquiry
APR12942G 100 μl / $495

PPP2R2B Antibody (Center)

Brand

Leading Biology

Catalog Number

APR12942G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PPP2R2B antibody (Center).

Molecular Weight

51710 Da

Cellular Localization

Antigen Cellular Localization: Isoform 1: Cytoplasm. Cytoplasm, cytoskeleton. Membrane

Host

Rabbit

Species Reactivity

Human

Immunogen

104-130 aa

Target

This PPP2R2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-130 amino acids from the Central region of human PPP2R2B.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Within the PP2A holoenzyme complex, isoform 2 is required to promote proapoptotic activity (By similarity). Isoform 2 regulates neuronal survival through the mitochondrial fission and fusion balance (By similarity).

Summary

PPP2R2B belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This protein is a beta isoform of the regulatory subunit B55 subfamily.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, E

Dilution

WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50

Synonyms

Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform, PP2A subunit B isoform B55-beta, PP2A subunit B isoform PR55-beta, PP2A subunit B isoform R2-beta, PP2A subunit B isoform beta, PPP2R2B

Images

Western blot analysis of PPP2R2B Antibody (Center) (Cat. APR12942G) in Hela cell line lysates (35ug/lane). PPP2R2B (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue with PPP2R2B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of hela cells using PPP2R2B Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specification

Quantity

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