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PIP5K1B Antibody (N-term)

Catalog # Availability Size / Price Inquiry
APR14298G 100 μl / $495

PIP5K1B Antibody (N-term)

Brand

Leading Biology

Catalog Number

APR14298G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PIP5K1B antibody (N-term).

Molecular Weight

61036 Da

Cellular Localization

Antigen Cellular Localization: Endomembrane system. Note=Associated with membranes.

Host

Rabbit

Species Reactivity

Human

Immunogen

1-30 aa

Target

This PIP5K1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PIP5K1B.

Isotype

Rabbit Ig

Symbol

STM7

GeneID

UniProt ID

Function

Participates in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. Mediates RAC1-dependent reorganization of actin filaments. Contributes to the activation of PLD2. Together with PIP5K1A is required after stimulation of G-protein coupled receptors for stable platelet adhesion (By similarity).

Summary

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, E

Dilution

WB~~1:1000 IHC-P~~1:50~100

Synonyms

Phosphatidylinositol 4-phosphate 5-kinase type-1 beta, PIP5K1-beta, PtdIns(4)P-5-kinase 1 beta, Phosphatidylinositol 4-phosphate 5-kinase type I beta, PIP5KIbeta, Protein STM-7, Type I phosphatidylinositol 4-phosphate 5-kinase beta, PIP5K1B, STM7

Images

All lanes : Anti-PIP5K1B Antibody (M1) at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of anti-PIP5K1B Pab (Cat. APR14298G) in human placenta cell lysate. PIP5K1B (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human brain tissue reacted with PIP5K1B antibody (N-term) (Cat. APR14298G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specification

Quantity

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