> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > Endophilin B1 Antibody (Y80)Brand |
Leading Biology | Catalog Number |
APR07690G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality Endophilin B1 antibody (Y80).
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Molecular Weight |
40796 Da
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Cellular Localization |
Antigen Cellular Localization:
Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Mitochondrion outer membrane; Peripheral membrane protein. Note=Association with the Golgi apparatus depends on the cell type.
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Host |
Rabbit
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Species Reactivity |
Human
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Immunogen |
59-86 aa
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Target |
This Endophilin B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-86 amino acids from human Endophilin B1.
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Isotype |
Rabbit Ig
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Symbol |
KIAA0491
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GeneID |
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UniProt ID |
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Function |
May be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells. May recruit other proteins to membranes with high curvature. May promote membrane fusion.
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Summary |
Endophilin B1 may be required for normal outer mitochondrial membrane dynamics. It is required for coatomer-mediated retrograde transport in certain cells. It may recruit other proteins to membranes with high curvature and may promote membrane fusion.
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Form |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IHC-P, E
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Dilution |
WB~~1:1000
IHC-P~~1:10~50
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Synonyms |
Endophilin-B1, Bax-interacting factor 1, Bif-1, SH3 domain-containing GRB2-like protein B1, SH3GLB1, KIAA0491
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Images |
Anti-Endophilin Antibody (Y80) at 1:1000 dilution + U118MG whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of anti-Endophilin B1 Antibody (Y80) (Cat. APR07690G) in HepG2 cell line lysates (35ug/lane). SH3GLB1 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Endophilin B1 Antibody (Y80) (Cat. APR07690G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Specification |
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Quantity |
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Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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