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Endophilin B1 Antibody (Y80)

Catalog # Availability Size / Price Inquiry
APR07690G 100 μl / $495

Endophilin B1 Antibody (Y80)

Brand

Leading Biology

Catalog Number

APR07690G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Endophilin B1 antibody (Y80).

Molecular Weight

40796 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Mitochondrion outer membrane; Peripheral membrane protein. Note=Association with the Golgi apparatus depends on the cell type.

Host

Rabbit

Species Reactivity

Human

Immunogen

59-86 aa

Target

This Endophilin B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-86 amino acids from human Endophilin B1.

Isotype

Rabbit Ig

Symbol

KIAA0491

GeneID

UniProt ID

Function

May be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells. May recruit other proteins to membranes with high curvature. May promote membrane fusion.

Summary

Endophilin B1 may be required for normal outer mitochondrial membrane dynamics. It is required for coatomer-mediated retrograde transport in certain cells. It may recruit other proteins to membranes with high curvature and may promote membrane fusion.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, E

Dilution

WB~~1:1000 IHC-P~~1:10~50

Synonyms

Endophilin-B1, Bax-interacting factor 1, Bif-1, SH3 domain-containing GRB2-like protein B1, SH3GLB1, KIAA0491

Images

Anti-Endophilin Antibody (Y80) at 1:1000 dilution + U118MG whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of anti-Endophilin B1 Antibody (Y80) (Cat. APR07690G) in HepG2 cell line lysates (35ug/lane). SH3GLB1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Endophilin B1 Antibody (Y80) (Cat. APR07690G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specification

Quantity

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