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cGKII Antibody (C-term)

Catalog # Availability Size / Price Inquiry
APR14283G 100 μl / $495

cGKII Antibody (C-term)

Brand

Leading Biology

Catalog Number

APR14283G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality cGKII antibody (C-term).

Molecular Weight

87432 Da

Cellular Localization

Antigen Cellular Localization: Apical cell membrane; Lipid-anchor

Host

Rabbit

Species Reactivity

Human

Immunogen

714-744 aa

Target

This cGKII antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 714-744 amino acids from the C-terminal region of human cGKII.

Isotype

Rabbit Ig

Symbol

PRKGR2

GeneID

UniProt ID

Summary

cGKII is thought to play a key role in a diverse set of physiological pathway. cGKII may mediate intestinal secretion of water and electrolytes induced by the E. coli toxin STa and the intestinal peptide guanylin. Edentification of the pathway that mediates intestinal fluid secretion by E. coli STa has potential medical implications because STa causes traveler's diarrhea and about 50% of infant mortality in developing countries. Transfection experiments in human cells disclose that cGKII phosphorylates SOX9 and attenuates SOX9 function by inhibiting its nuclear entry. Impaired differentiation of cultured KMI chondrocytes can be restored by silencing Sox9 by RNA interference. cGKII is postulated to be a molecular switch that couples the cessation of proliferation and the start of hypertrophic chondrocyte differentiation through attenuating SOX9 function.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, E

Dilution

WB~~1:1000 IHC-P~~1:50~100

Synonyms

cGMP-dependent protein kinase 2, cGK 2, cGK2, cGMP-dependent protein kinase II, cGKII, PRKG2, PRKGR2

Images

Western blot analysis of cGKII (arrow) using cGKII Antibody (C-term) (Cat. APR14283G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKG2 gene (Lane 2).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with cGKII antibody (C-term) (Cat. APR14283G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specification

Quantity

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