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CYP2W1 Antibody (N-term)

APG02905G Product Datasheet Protocol

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APG02905G		100 μl / $495

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Leading Biology Inc.

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CYP2W1 Antibody (N-term)

Brand	Leading Biology	Catalog Number	APG02905G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CYP2W1 antibody (N-term).
Molecular Weight	53844 Da
Cellular Localization	Antigen Cellular Localization: Endoplasmic reticulum lumen. Cell membrane. Note=About 8% are expressed on the cell surface.
Host	Rabbit
Species Reactivity	Human
Immunogen	7-33 aa
Target	This CYP2W1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-33 amino acids from the N-terminal region of human CYP2W1.
Isotype	Rabbit Ig
GeneID	54905
UniProt ID	Q8TAV3
Function	Seems to have broad catalytic activity towards several chemicals, including polycyclic aromatic hydrocarbon dihydrodiols and aromatic amines (PubMed:16551781, PubMed:24278521). Actives also in the metabolism of indoline substrates and is able to activate aflatoxin B1 into cytotoxic products (PubMed:20805301). Furthermore, it seems to be involved in the oxydation of lysophospholipids and fatty acids (PubMed:22591743).
Summary	CYP2W1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, E
Dilution	WB~~1:1000 IHC-P~~1:10~50
Synonyms	Cytochrome P450 2W1, 11414-, CYPIIW1, CYP2W1
Images	Western blot analysis of lysates from MCF-7, HepG2 cell line (from left to right), using CYP2W1 Antibody (N-term)( APG02905G). APG02905G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane. Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CYP2W1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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