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CYP20A1 Antibody (C-term)

APG02859G Product Datasheet Protocol

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APG02859G		100 μl / $495

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Leading Biology Inc.

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CYP20A1 Antibody (C-term)

Brand	Leading Biology	Catalog Number	APG02859G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CYP20A1 antibody (C-term).
Molecular Weight	52432 Da
Cellular Localization	Antigen Cellular Localization: Membrane; Single-pass membrane protein
Host	Rabbit
Species Reactivity	Human
Immunogen	364-392 aa
Target	This CYP20A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364-392 amino acids from the C-terminal region of human CYP20A1.
Isotype	Rabbit Ig
GeneID	57404
UniProt ID	Q6UW02
Summary	CYP20A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein lacks one amino acid of the conserved heme binding site. It also lacks the conserved I-helix motif AGX(D,E)T, suggesting that its substrate may carry its own oxygen.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, FC, E
Dilution	WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50
Synonyms	Cytochrome P450 20A1, 114--, CYP20A1
Images	Western blot analysis of CYP20A1(arrow) using rabbit polyclonal CYP20A1 Antibody (C-term)(Cat. APG02859G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CYP20A1 gene (Lane 2) (Origene Technologies). Formalin-fixed and paraffin-embedded human hepatocarcinoma with CYP20A1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Flow cytometric analysis of NCI-H292 cells using CYP20A1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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