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COG4 Antibody (C-term)

APR15530G Product Datasheet Protocol

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APR15530G		100 μl / $495

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Leading Biology Inc.

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COG4 Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR15530G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality COG4 antibody (C-term).
Molecular Weight	89083 Da
Cellular Localization	Antigen Cellular Localization: Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side
Host	Rabbit
Species Reactivity	Human
Immunogen	757-785 aa
Target	This COG4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 757-785 amino acids from the C-terminal region of human COG4.
Isotype	Rabbit Ig
GeneID	25839
UniProt ID	Q9H9E3
Function	Required for normal Golgi function. Plays a role in SNARE-pin assembly and Golgi-to-ER retrograde transport via its interaction with SCFD1.
Summary	Multiprotein complexes are key determinants of Golgi apparatus structure and its capacity for intracellular transport and glycoprotein modification. Several complexes have been identified, including the Golgi transport complex (GTC), the LDLC complex, which is involved in glycosylation reactions, and the SEC34 complex, which is involved in vesicular transport. These 3 complexes are identical and have been termed the conserved oligomeric Golgi (COG) complex, which includes COG4.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, FC, E
Dilution	WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50
Synonyms	Conserved oligomeric Golgi complex subunit 4, COG complex subunit 4, Component of oligomeric Golgi complex 4, COG4
Images	Anti-COG4 Antibody (C-term) at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 89 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of COG4 Antibody (C-term) (Cat. APR15530G) in A2058 cell line lysates (35ug/lane). COG4 (arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded human skeletal muscle with COG4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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