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CKM Antibody (N-term)

Catalog # Availability Size / Price Inquiry
APG02638G 100 μl / $495

CKM Antibody (N-term)

Brand

Leading Biology

Catalog Number

APG02638G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CKM antibody (N-term).

Molecular Weight

43101 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

1-30 aa

Target

This CKM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CKM.

Isotype

Rabbit Ig

Symbol

CKMM

GeneID

UniProt ID

Function

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Summary

Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. The CKM isoform, predominant in skeletal muscle and heart tissue, is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. CKM reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, E

Dilution

WB~~1:1000

Synonyms

Creatine kinase M-type, Creatine kinase M chain, M-CK, Creatine kinase M-type, N-terminally processed, CKM, CKMM

Images

The anti-CKM Pab (Cat. APG02638G) is used in Western blot to detect CKM in mouse heart tissue lysate.

Western blot analysis of CKM (arrow) using rabbit polyclonal CKM-T6 (Cat. APG02638G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CKM gene.

Specification

Quantity

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