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ACTG1 Antibody (Center)

Catalog # Availability Size / Price Inquiry
AMM05544G 100 μl / $495

ACTG1 Antibody (Center)

Brand

Leading Biology

Catalog Number

AMM05544G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ACTG1 antibody (Center).

Molecular Weight

41793 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm, cytoskeleton.

Host

Rabbit

Species Reactivity

Human

Immunogen

188-215 aa

Target

This ACTG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-215 amino acids from the Central region of human ACTG1.

Isotype

Rabbit Ig

Symbol

ACTG

GeneID

UniProt ID

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Summary

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, is a cytoplasmic actin found in nonmuscle cells.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, FC, IF, E

Dilution

WB~~1:1000 FC~~1:10~50 IF~~1:10~50

Synonyms

Actin, cytoplasmic 2, Gamma-actin, Actin, cytoplasmic 2, N-terminally processed, ACTG1, ACTG

Images

Western blot analysis of ACTG1 Antibody (Center) (Cat. AMM05544G) in K562, HepG2 cell line lysates (35ug/lane). ACTG1 (arrow) was detected using the purified Pab.

Flow cytometric analysis of K562 cells using ACTG1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of ACTG1 (arrow) using rabbit polyclonal ACTG1 Antibody (Center) (Cat. AMM05544G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ACTG1 gene.

Specification

Quantity

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