> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > Leptin (LEP) Antibody (C-term)Brand |
Leading Biology | Catalog Number |
APR08193G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality Leptin (LEP) antibody (C-term).
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Molecular Weight |
18641 Da
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Cellular Localization |
Antigen Cellular Localization:
Secreted.
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Host |
Rabbit
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Species Reactivity |
Human
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Immunogen |
134-166 aa
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Target |
This Leptin (LEP) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-166 amino acids from the C-terminal region of human Leptin (LEP).
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Isotype |
Rabbit Ig
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Symbol |
OB, OBS
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GeneID |
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UniProt ID |
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Function |
May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
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Summary |
Leptin (LEP) is an adipocyte-secreted protein that regulates body fat and endocrine functions. Leptin is similar to the mouse obesity protein encoded by the obese gene (ob). In mouse studies, mutations in this gene are linked to severe and morbid obesity.
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Form |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IF, E
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Dilution |
WB~~1:1000
IF~~1:10~50
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Synonyms |
Leptin, Obese protein, Obesity factor, LEP, OB, OBS
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Images |
Western blot analysis of hLep-R149 (Cat. APR08193G) in MDA-MB231 cell line lysates (35ug/lane). LEP (arrow) was detected using the purified Pab.
Confocal immunofluorescent analysis of Leptin (LEP) Antibody (C-term)( APR08193G) with HepG2 cell followed by Alexa Fluor? 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). |
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Specification |
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Quantity |
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Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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