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Leptin (LEP) Antibody (C-term)

Catalog # Availability Size / Price Inquiry
APR08193G 100 μl / $495

Leptin (LEP) Antibody (C-term)

Brand

Leading Biology

Catalog Number

APR08193G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Leptin (LEP) antibody (C-term).

Molecular Weight

18641 Da

Cellular Localization

Antigen Cellular Localization: Secreted.

Host

Rabbit

Species Reactivity

Human

Immunogen

134-166 aa

Target

This Leptin (LEP) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-166 amino acids from the C-terminal region of human Leptin (LEP).

Isotype

Rabbit Ig

Symbol

OB, OBS

GeneID

UniProt ID

Function

May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.

Summary

Leptin (LEP) is an adipocyte-secreted protein that regulates body fat and endocrine functions. Leptin is similar to the mouse obesity protein encoded by the obese gene (ob). In mouse studies, mutations in this gene are linked to severe and morbid obesity.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IF, E

Dilution

WB~~1:1000 IF~~1:10~50

Synonyms

Leptin, Obese protein, Obesity factor, LEP, OB, OBS

Images

Western blot analysis of hLep-R149 (Cat. APR08193G) in MDA-MB231 cell line lysates (35ug/lane). LEP (arrow) was detected using the purified Pab.

Confocal immunofluorescent analysis of Leptin (LEP) Antibody (C-term)( APR08193G) with HepG2 cell followed by Alexa Fluor? 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Specification

Quantity

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