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ABCB10 Antibody (N-term)

APG01404G Product Datasheet Protocol

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APG01404G		100 μl / $495

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Leading Biology Inc.

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ABCB10 Antibody (N-term)

Brand	Leading Biology	Catalog Number	APG01404G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ABCB10 antibody (N-term).
Molecular Weight	79148 Da
Cellular Localization	Antigen Cellular Localization: Mitochondrion inner membrane; Multi-pass membrane protein
Host	Rabbit
Species Reactivity	Human
Immunogen	1-27 aa
Target	This ABCB10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-27 amino acids from the N-terminal region of human ABCB10.
Isotype	Rabbit Ig
GeneID	23456
UniProt ID	Q9NRK6
Function	May mediate critical mitochondrial transport functions related to heme biosynthesis.
Summary	The membrane-associated protein ABCB10 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The function of this mitochondrial protein is unknown.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, E
Dilution	IHC-P~~1:10~50 WB~~1:1000
Synonyms	ATP-binding cassette sub-family B member 10, mitochondrial, ATP-binding cassette transporter 10, ABC transporter 10 protein, Mitochondrial ATP-binding cassette 2, M-ABC2, ABCB10
Images	APG01404G staining hABCB10 in Human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. All lanes : Anti-ABCB10 Antibody (S16) at 1:1000-1:2000 dilution Lane 1: K562 whole cell lysates Lane 2: Raji whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 79 kDa Blocking/Dilution buffer: 5% NFDM/TBST. ABCB10 Antibody (S16) (Cat. APG01404G) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the ABCB10 antibody detected the ABCB10 protein (arrow).
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