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CLTC Antibody (Center)

Catalog # Availability Size / Price Inquiry
APR15524G 100 μl / $495

CLTC Antibody (Center)

Brand

Leading Biology

Catalog Number

APR15524G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CLTC antibody (Center).

Molecular Weight

191615 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Melanosome. Note=Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV

Host

Rabbit

Species Reactivity

Human

Immunogen

1019-1048 aa

Target

This CLTC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1019-1048 amino acids from the Central region of human CLTC.

Isotype

Rabbit Ig

Symbol

CLH17, CLTCL2, KIAA0034

GeneID

UniProt ID

Function

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, E

Dilution

WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50

Synonyms

Clathrin heavy chain 1, Clathrin heavy chain on chromosome 17, CLH-17, CLTC, CLH17, CLTCL2, KIAA0034

Images

Anti-CLTC Antibody (Center) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 192 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

CLTC antibody (Center) (Cat. APR15524G) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CLTC antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

CLTC Antibody (Center) (Cat. APR15524G) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specification

Quantity

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