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DNAJA1 Antibody (Center)

Catalog # Availability Size / Price Inquiry
APR15758G 100 μl / $495

DNAJA1 Antibody (Center)

Brand

Leading Biology

Catalog Number

APR15758G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality DNAJA1 antibody (Center).

Molecular Weight

44868 Da

Cellular Localization

Antigen Cellular Localization: Membrane; Lipid-anchor. Cytoplasm. Microsome. Nucleus. Cytoplasm, perinuclear region. Mitochondrion Note=Primarily associated with microsomes. A minor proportion is associated with mitochondria (By similarity). Primarily cytoplasmic. A minor proportion is associated with nuclei

Host

Rabbit

Species Reactivity

Human

Immunogen

294-323 aa

Target

This DNAJA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 294-323 amino acids from the Central region of human DNAJA1.

Isotype

Rabbit Ig

Symbol

DNAJ2, HDJ2, HSJ2, HSPF4

GeneID

UniProt ID

Function

Co-chaperone for HSPA8/Hsc70 (PubMed:10816573). Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro) (PubMed:24318877). Plays a role in protein transport into mitochondria via its role as co- chaperone. Functions as co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis (PubMed:14752510). Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV (PubMed:24512202).

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, E

Dilution

WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50

Synonyms

DnaJ homolog subfamily A member 1, DnaJ protein homolog 2, HSDJ, Heat shock 40 kDa protein 4, Heat shock protein J2, HSJ-2, Human DnaJ protein 2, hDj-2, DNAJA1, DNAJ2, HDJ2, HSJ2, HSPF4

Images

DNAJA1 Antibody (Center) (Cat. APR15758G) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the DNAJA1 antibody detected the DNAJA1 protein (arrow).

DNAJA1 Antibody (Center) (Cat. APR15758G) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DNAJA1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Western blot analysis of DNAJA1 (arrow) using rabbit polyclonal DNAJA1 Antibody (Center) (Cat. APR15758G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DNAJA1 gene.

Specification

Quantity

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