> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > PACAP-27/38 AntibodyBrand |
Leading Biology | Catalog Number |
APR12700G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality PACAP-27/38 antibody.
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Molecular Weight |
19; [uniprot]19; [RD]19; KDa
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Cellular Localization |
Antigen Cellular Localization:
Secreted.
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Host |
Rabbit
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Species Reactivity |
Human, Mouse, Rat
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Immunogen |
132-169/176 aa
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GeneID |
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UniProt ID |
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Function |
Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells. Promotes neuron projection development through the RAPGEF2/Rap1/B-Raf/ERK pathway.
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Summary |
Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells. Promotes neuron projection development through the RAPGEF2/Rap1/B-Raf/ERK pathway.
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Form |
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce |
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IHC
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Dilution |
WB~~ 1:500
IHC~~1:100-1:500
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Synonyms |
Pituitary adenylate cyclase-activating polypeptide, PACAP, PACAP-related peptide, PRP-48, Pituitary adenylate cyclase-activating polypeptide 27, PACAP-27, PACAP27, Pituitary adenylate cyclase-activating polypeptide 38, PACAP-38, PACAP38, ADCYAP1
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Images |
Western blot analysis of lysate from human brain tissue lysate,using PACAP-27/38 Antibody(APR12700G). APR12700G was diluted at 1:500. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug.
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-PACAP-27/38 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining |
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Specification |
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Quantity |
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Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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