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PACAP-27/38 Antibody

Catalog # Availability Size / Price Inquiry
APR12700G 100 μl / $495

PACAP-27/38 Antibody

Brand

Leading Biology

Catalog Number

APR12700G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PACAP-27/38 antibody.

Molecular Weight

19; [uniprot]19; [RD]19; KDa

Cellular Localization

Antigen Cellular Localization: Secreted.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

132-169/176 aa

GeneID

UniProt ID

Function

Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells. Promotes neuron projection development through the RAPGEF2/Rap1/B-Raf/ERK pathway.

Summary

Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells. Promotes neuron projection development through the RAPGEF2/Rap1/B-Raf/ERK pathway.

Form

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC

Dilution

WB~~ 1:500 IHC~~1:100-1:500

Synonyms

Pituitary adenylate cyclase-activating polypeptide, PACAP, PACAP-related peptide, PRP-48, Pituitary adenylate cyclase-activating polypeptide 27, PACAP-27, PACAP27, Pituitary adenylate cyclase-activating polypeptide 38, PACAP-38, PACAP38, ADCYAP1

Images

Western blot analysis of lysate from human brain tissue lysate,using PACAP-27/38 Antibody(APR12700G). APR12700G was diluted at 1:500. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug.

Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-PACAP-27/38 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

Specification

Quantity

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