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PLA2G1B Antibody (C-term)

Catalog # Availability Size / Price Inquiry
AMR09317G 100 μl / $495

PLA2G1B Antibody (C-term)

Brand

Leading Biology

Catalog Number

AMR09317G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PLA2G1B antibody (C-term).

Molecular Weight

16360 Da

Cellular Localization

Antigen Cellular Localization: Secreted. Note=secreted from pancreatic acinar cells in its inactive form

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

116-145 aa

Target

This PLA2G1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-145 amino acids from the C-terminal region of human PLA2G1B.

Isotype

Rabbit Ig

Symbol

PLA2, PLA2A, PPLA2

GeneID

UniProt ID

Function

PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides, this releases glycerophospholipids and arachidonic acid that serve as the precursors of signal molecules.

Summary

PLA2G1B catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic PLA2 enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of PLA2 have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, FC, E

Dilution

WB~~1:1000 FC~~1:10~50

Synonyms

Phospholipase A2, Group IB phospholipase A2, Phosphatidylcholine 2-acylhydrolase 1B, PLA2G1B, PLA2, PLA2A, PPLA2

Images

Western blot analysis of PLA2G1B Antibody (C-term) (Cat. AMR09317G) in mouse spleen tissue lysates (35ug/lane). PLA2G1B (arrow) was detected using the purified Pab.

Western blot analysis of PLA2G1B (arrow) using rabbit polyclonal PLA2G1B Antibody (C-term) (Cat. AMR09317G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PLA2G1B gene.

PLA2G1B Antibody (C-term) (Cat. AMR09317G) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specification

Quantity

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