> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > CCT3 Antibody (C-term)Brand |
Leading Biology | Catalog Number |
APR15319G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality CCT3 antibody (C-term).
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Molecular Weight |
60534 Da
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Cellular Localization |
Antigen Cellular Localization:
Cytoplasm.
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Host |
Rabbit
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Species Reactivity |
Human
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Immunogen |
509-536 aa
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Target |
This CCT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 509-536 amino acids from the C-terminal region of human CCT3.
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Isotype |
Rabbit Ig
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Symbol |
CCTG, TRIC5
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GeneID |
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UniProt ID |
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Function |
Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
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Summary |
CCT3 is a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin.
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Form |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IHC-P, FC, E
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Dilution |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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Synonyms |
T-complex protein 1 subunit gamma, TCP-1-gamma, CCT-gamma, hTRiC5, CCT3, CCTG, TRIC5
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Images |
Western blot analysis of CCT3 Antibody (C-term) (Cat. APR15319G) in K562 cell line lysates (35ug/lane). CCT3 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CCT3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of K562 cells using CCT3 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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Specification |
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Quantity |
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Leading Biology Inc.
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Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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