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AKR1A1 Antibody (C-term)

Catalog # Availability Size / Price Inquiry
APG01646G 100 μl / $495

AKR1A1 Antibody (C-term)

Brand

Leading Biology

Catalog Number

APG01646G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality AKR1A1 antibody (C-term).

Molecular Weight

36573 Da

Host

Rabbit

Species Reactivity

Human

Immunogen

293-325 aa

Target

This AKR1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 293-325 amino acids from the C-terminal region of human AKR1A1.

Isotype

Rabbit Ig

Symbol

ALDR1, ALR

GeneID

UniProt ID

Function

Catalyzes the NADPH-dependent reduction of a variety of aromatic and aliphatic aldehydes to their corresponding alcohols. Catalyzes the reduction of mevaldate to mevalonic acid and of glyceraldehyde to glycerol. Has broad substrate specificity. In vitro substrates include succinic semialdehyde, 4- nitrobenzaldehyde, 1,2-naphthoquinone, methylglyoxal, and D- glucuronic acid. Plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs, including the anthracyclines doxorubicin (DOX) and daunorubicin (DAUN).

Summary

AKR1A1 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member, also known as aldehyde reductase, is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

IHC-P, WB, E

Dilution

IHC-P~~1:10~50 WB~~1:1000

Synonyms

Alcohol dehydrogenase [NADP(+)], Aldehyde reductase, Aldo-keto reductase family 1 member A1, AKR1A1, ALDR1, ALR

Images

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AKR1A1 antibody (C-term) (Cat. APG01646G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of anti-AKR1A1 Antibody (C-term) (Cat. APG01646G) in Y79 cell line lysates (35ug/lane). AKR1A1(arrow) was detected using the purified Pab.

Western blot analysis of AKR1A1 (arrow) using rabbit polyclonal AKR1A1 Antibody (C-term) (Cat. APG01646G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the AKR1A1 gene.

Specification

Quantity

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