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MSX1 Antibody (Center)

AMM06496G Product Datasheet Protocol

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AMM06496G		100 μl / $495

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Leading Biology Inc.

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MSX1 Antibody (Center)

Brand	Leading Biology	Catalog Number	AMM06496G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality MSX1 antibody (Center).
Molecular Weight	31496 Da
Cellular Localization	Antigen Cellular Localization: Nucleus.
Host	Rabbit
Species Reactivity	Human, Mouse
Immunogen	111-138 aa
Target	This MSX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-138 amino acids from the Central region of human MSX1.
Isotype	Rabbit Ig
Symbol	HOX7
GeneID	4487
UniProt ID	P28360
Function	Acts as a transcriptional repressor. May play a role in limb-pattern formation. Acts in cranofacial development and specifically in odontogenesis. Expression in the developing nail bed mesenchyme is important for nail plate thickness and integrity.
Summary	This gene encodes a member of the muscle segment homeoboxgene family. The encoded protein functions as a transcriptionalrepressor during embryogenesis through interactions with componentsof the core transcription complex and other homeoproteins. It mayalso have roles in limb-pattern formation, craniofacialdevelopment, particularly odontogenesis, and tumor growthinhibition. Mutations in this gene, which was once known ashomeobox 7, have been associated with nonsyndromic cleft lip withor without cleft palate 5, Witkop syndrome, Wolf-Hirschom syndrome,and autosomoal dominant hypodontia.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	IF, WB, E
Dilution	WB~~1:1000 IF~~1:200
Synonyms	Homeobox protein MSX-1, Homeobox protein Hox-7, Msh homeobox 1-like protein, MSX1, HOX7
Images	Anti-MSX1 Antibody (Center)at 1:2000 dilution + mouse spleen lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Fluorescent image of U251 cell stained with MSX1 Antibody (Center)( AMM06496G).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MSX1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).MSX1 immunoreactivity is localized to Nucleus significantly. MSX1 Antibody (Center)(Cat. AMM06496G) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the MSX1 antibody detected the MSX1 protein (arrow).
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