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Connexin 36 Antibody (C-term)

Catalog # Availability Size / Price Inquiry
APR15548G 100 μl / $495

Connexin 36 Antibody (C-term)

Brand

Leading Biology

Catalog Number

APR15548G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Connexin 36 antibody (C-term).

Molecular Weight

36093 Da

Cellular Localization

Antigen Cellular Localization: Cell membrane; Multi-pass membrane protein. Cell junction, gap junction

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

291-321 aa

Target

This Connexin 36 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 291-321 amino acids from the C-terminal region of human Connexin 36.

Isotype

Rabbit Ig

Symbol

GJA9

GeneID

UniProt ID

Function

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

Summary

GJA9, also called connexin-36 (CX36), is a member of the connexin gene family that is expressed predominantly in mammalian neurons. Connexins associate in groups of 6 and are organized radially around a central pore to form connexons. Each gap junction intercellular channel is formed by the conjunction of 2 connexons.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, E

Dilution

WB~~1:2000 IHC-P~~1:50~100

Synonyms

Gap junction delta-2 protein, Connexin-36, Cx36, Gap junction alpha-9 protein, GJD2, GJA9

Images

All lanes : Anti-Connexin 36 Antibody (C-term) at 1:2000 dilution Lane 1: human brain lysate Lane 2: mouse brain lysate Lane 3: rat brain lysate Lane 4: SH-SY5Y whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Specification

Quantity

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