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PYGM Antibody (C-term)

AMR09673G Product Datasheet Protocol

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AMR09673G		100 μl / $495

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Leading Biology Inc.

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PYGM Antibody (C-term)

Brand	Leading Biology	Catalog Number	AMR09673G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PYGM antibody (C-term).
Molecular Weight	97092 Da
Host	Rabbit
Species Reactivity	Human
Immunogen	698-727 aa
Target	This PYGM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 698-727 amino acids from the C-terminal region of human PYGM.
Isotype	Rabbit Ig
GeneID	5837
UniProt ID	P11217
Function	Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.
Summary	PYGM catalyzes and regulates the breakdown of glycogen to glucose-1-phosphate. Defects in PYGM are the cause of glycogen storage disease type 5 (GSD5), also known as McArdle disease. GSD5 is a metabolic disorder resulting in myopathy characterized by exercise intolerance, cramps, muscle weakness and recurrent myoglobinuria.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, E
Dilution	WB~~1:1000 IHC-P~~1:10~50
Synonyms	Glycogen phosphorylase, muscle form, Myophosphorylase, PYGM
Images	Anti-PYGM Antibody (C-term) at 1:8000 dilution + human skeletal muscle lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 97 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of PYGM (arrow) using rabbit polyclonal PYGM Antibody (C-term) (Cat. AMR09673G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PYGM gene (Lane 2) (Origene Technologies). Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PYGM antibody (C-term) (Cat. AMR09673G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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