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VASP Antibody (C-term)

AMM08457G Product Datasheet Protocol

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AMM08457G		100 μl / $495

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Leading Biology Inc.

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VASP Antibody (C-term)

Brand	Leading Biology	Catalog Number	AMM08457G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality VASP antibody (C-term).
Molecular Weight	39830 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm. Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cell junction, tight junction. Cell projection, lamellipodium membrane. Cell projection, filopodium membrane. Note=Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members. Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions Localized along the sides of actin filaments throughout the peripheral cytoplasm under basal conditions. In pre-apoptotic cells, colocalizes with MEFV in large specks (pyroptosomes)
Host	Rabbit
Species Reactivity	Human
Immunogen	267-296 aa
Target	This VASP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 267-296 amino acids from the C-terminal region of human VASP.
Isotype	Rabbit Ig
GeneID	7408
UniProt ID	P50552
Function	Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin- bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
Summary	Vasodilator-stimulated phosphoprotein (VASP) is a memberof the Ena-VASP protein family. Ena-VASP family members contain anEHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/Emotifs and targets Ena-VASP proteins to focal adhesions. In themid-region of the protein, family members have a proline-richdomain that binds SH3 and WW domain-containing proteins. TheirC-terminal EVH2 domain mediates tetramerization and binds both Gand F actin. VASP is associated with filamentous actin formationand likely plays a widespread role in cell adhesion and motility.VASP may also be involved in the intracellular signaling pathwaysthat regulate integrin-extracellular matrix interactions. VASP isregulated by the cyclic nucleotide-dependent kinases PKA and PKG.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, E
Dilution	WB~~1:2000 IHC-P~~1:10~50
Synonyms	Vasodilator-stimulated phosphoprotein, VASP, VASP
Images	All lanes : Anti-VASP Antibody (C-term) at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: HUVEC whole cell lysate Lane 5: MDA-MB-453 whole cell lysate Lane 6: THP-1 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST. VASP Antibody (C-term) (Cat. AMM08457G)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of VASP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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