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EHD1 Antibody (C-term)

Catalog # Availability Size / Price Inquiry
APR07647G 100 μl / $495

EHD1 Antibody (C-term)

Brand

Leading Biology

Catalog Number

APR07647G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality EHD1 antibody (C-term).

Molecular Weight

60627 Da

Cellular Localization

Antigen Cellular Localization: Recycling endosome membrane; Peripheral membrane protein. Early endosome membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Preferentially associates with tubular recycling endosomes. Colocalizes with FER1L5 at plasma membrane in myoblasts and myotubes

Host

Rabbit

Species Reactivity

Human

Immunogen

410-438 aa

Target

This EHD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 410-438 amino acids from the C-terminal region of human EHD1.

Isotype

Rabbit Ig

Symbol

PAST, PAST1

GeneID

UniProt ID

Function

Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Plays a role in myoblast fusion. Recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth.

Summary

This gene belongs to a highly conserved gene familyencoding EPS15 homology (EH) domain-containing proteins. Theprotein-binding EH domain was first noted in EPS15, a substrate forthe epidermal growth factor receptor. The EH domain has been shownto be an important motif in proteins involved in protein-proteininteractions and in intracellular sorting. The protein encoded bythis gene is thought to play a role in the endocytosis of IGF1receptors.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, E

Dilution

WB~~1:1000

Synonyms

EH domain-containing protein 1, PAST homolog 1, hPAST1, Testilin, EHD1, PAST, PAST1

Images

All lanes : Anti-EHD1 Antibody (C-term) at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 61kDa Blocking/Dilution buffer: 5% NFDM/TBST.

EHD1 Antibody (C-term) (Cat. APR07647G) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the EHD1 antibody detected the EHD1 protein (arrow).

Specification

Quantity

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