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NETO2 Antibody (N-term )

AMM06612G Product Datasheet Protocol

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AMM06612G		100 μl / $495

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NETO2 Antibody (N-term )

Brand	Leading Biology	Catalog Number	AMM06612G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality NETO2 antibody (N-term ).
Molecular Weight	59393 Da
Cellular Localization	Antigen Cellular Localization: Membrane; Single-pass type I membrane protein
Host	Rabbit
Species Reactivity	Human
Immunogen	174-203 aa
Target	This NETO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 174-203 amino acids from the N-terminal region of human NETO2.
Isotype	Rabbit Ig
Symbol	BTCL2
GeneID	81831
UniProt ID	Q8NC67
Function	Accessory subunit of neuronal kainate-sensitive glutamate receptors, GRIK2 and GRIK3. Increases kainate-receptor channel activity, slowing the decay kinetics of the receptors, without affecting their expression at the cell surface, and increasing the open probability of the receptor channels. Modulates the agonist sensitivity of kainate receptors. Slows the decay of kainate receptor-mediated excitatory postsynaptic currents (EPSCs), thus directly influencing synaptic transmission (By similarity).
Summary	This gene encodes a predicted transmembrane proteincontaining two extracellular CUB domains followed by a low-densitylipoprotein class A (LDLa) domain. It also has an intracellularFXNPXY-like motif, which has been shown in other proteins to beessential for the internalization of clathrin coated pits duringendocytosis. Alternatively spliced transcript variants have beenobserved, but they have not been fully characterized. [provided byRefSeq].
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	FC, WB, IHC-P, E
Dilution	FC~~1:25 WB~~1:2000 IHC-P~~1:10~50
Synonyms	Neuropilin and tolloid-like protein 2, Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 2, NETO2, BTCL2
Images	Overlay histogram showing Jurkat cells stained with AMM06612G (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AMM06612G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. Anti-NETO2 Antibody (N-term ) at 1:2000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 59 kDa Blocking/Dilution buffer: 5% NFDM/TBST. NETO2 Antibody (N-term ) (Cat. AMM06612G)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NETO2 Antibody (N-term ) for immunohistochemistry. Clinical relevance has not been evaluated.
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