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KRT12 Antibody (C-term)

AMM06212G Product Datasheet Protocol

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AMM06212G		100 μl / $495

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Leading Biology Inc.

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KRT12 Antibody (C-term)

Brand	Leading Biology	Catalog Number	AMM06212G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality KRT12 antibody (C-term).
Molecular Weight	53511 Da
Host	Rabbit
Species Reactivity	Human, Mouse, Rat
Immunogen	442-471 aa
Target	This KRT12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 442-471 amino acids from the C-terminal region of human KRT12.
Isotype	Rabbit Ig
GeneID	3859
UniProt ID	Q99456
Function	May play a unique role in maintaining the normal corneal epithelial function. Together with KRT3, essential for the maintenance of corneal epithelium integrity (By similarity).
Summary	KRT12 encodes the type I intermediate filament chainkeratin 12, expressed in corneal epithelia. Mutations in this genelead to Meesmann corneal dystrophy.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, FC, E
Dilution	FC~~1:25 WB~~1:1000 IHC-P~~1:10~50
Synonyms	Keratin, type I cytoskeletal 12, Cytokeratin-12, CK-12, Keratin-12, K12, KRT12
Images	Overlay histogram showing K562 cells stained with AMM06212G (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AMM06212G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. Anti-KRT12 Antibody (C-term) at 1:2000 dilution + rat eyeball lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Anti-KRT12 Antibody (C-term)at 1:2000 dilution + mouse eyeball lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 53 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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