> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > SDHD Antibody (N-term)Brand |
Leading Biology | Catalog Number |
AMR09852G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality SDHD antibody (N-term).
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Molecular Weight |
17043 Da
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Cellular Localization |
Antigen Cellular Localization:
Mitochondrion inner membrane; Multi-pass membrane protein
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Host |
Rabbit
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Species Reactivity |
Human
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Immunogen |
13-42 aa
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Target |
This SDHD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human SDHD.
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Isotype |
Rabbit Ig
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Symbol |
SDH4
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GeneID |
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UniProt ID |
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Function |
Membrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
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Summary |
Complex II of the respiratory chain, which isspecifically involved in the oxidation of succinate, carrieselectrons from FADH to CoQ. The complex is composed of fournuclear-encoded subunits and is localized in the mitochondrialinner membrane. The subunit D protein is one of two integralmembrane proteins anchoring the complex to the matrix side of themembrane. Mutations in SDHD have been linked to hereditaryparaganglioma.
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Form |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IHC-P, IF, FC, E
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Dilution |
WB~~1:1000
IHC-P~~1:10~50
IF~~1:10~50
FC~~1:10~50
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Synonyms |
Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial, CybS, CII-4, QPs3, Succinate dehydrogenase complex subunit D, Succinate-ubiquinone oxidoreductase cytochrome b small subunit, Succinate-ubiquinone reductase membrane anchor subunit, SDHD, SDH4
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Images |
All lanes : Anti-SDHD Antibody (N-term) at 1:2000 dilution Lane 1: A2058 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 17 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
SDHD Antibody (N-term) (Cat. AMR09852G) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SDHD antibody detected the SDHD protein (arrow).
SDHD Antibody (N-term) (Cat. AMR09852G)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SDHD Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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