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SDHD Antibody (N-term)

Catalog # Availability Size / Price Inquiry
AMR09852G 100 μl / $495

SDHD Antibody (N-term)

Brand

Leading Biology

Catalog Number

AMR09852G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality SDHD antibody (N-term).

Molecular Weight

17043 Da

Cellular Localization

Antigen Cellular Localization: Mitochondrion inner membrane; Multi-pass membrane protein

Host

Rabbit

Species Reactivity

Human

Immunogen

13-42 aa

Target

This SDHD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human SDHD.

Isotype

Rabbit Ig

Symbol

SDH4

GeneID

UniProt ID

Function

Membrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).

Summary

Complex II of the respiratory chain, which isspecifically involved in the oxidation of succinate, carrieselectrons from FADH to CoQ. The complex is composed of fournuclear-encoded subunits and is localized in the mitochondrialinner membrane. The subunit D protein is one of two integralmembrane proteins anchoring the complex to the matrix side of themembrane. Mutations in SDHD have been linked to hereditaryparaganglioma.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, IF, FC, E

Dilution

WB~~1:1000 IHC-P~~1:10~50 IF~~1:10~50 FC~~1:10~50

Synonyms

Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial, CybS, CII-4, QPs3, Succinate dehydrogenase complex subunit D, Succinate-ubiquinone oxidoreductase cytochrome b small subunit, Succinate-ubiquinone reductase membrane anchor subunit, SDHD, SDH4

Images

All lanes : Anti-SDHD Antibody (N-term) at 1:2000 dilution Lane 1: A2058 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 17 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

SDHD Antibody (N-term) (Cat. AMR09852G) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SDHD antibody detected the SDHD protein (arrow).

SDHD Antibody (N-term) (Cat. AMR09852G)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SDHD Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Specification

Quantity

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