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EDN1 Antibody (C-term)

APR07642G Product Datasheet Protocol

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APR07642G		100 μl / $495

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Leading Biology Inc.

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EDN1 Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR07642G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality EDN1 antibody (C-term).
Molecular Weight	24425 Da
Cellular Localization	Antigen Cellular Localization: Secreted.
Host	Rabbit
Species Reactivity	Human
Immunogen	176-203 aa
Target	This EDN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-203 amino acids from the C-terminal region of human EDN1.
Isotype	Rabbit Ig
GeneID	1906
UniProt ID	P05305
Function	Endothelins are endothelium-derived vasoconstrictor peptides.
Summary	The protein encoded by this gene is proteolyticallyprocessed to release a secreted peptide termed endothelin 1. Thispeptide is a potent vasoconstrictor and is produced by vascularendothelial cells. Endothelin 1 also can affect the central nervoussystem. Two transcript variants encoding different isoforms havebeen found for this gene.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	IHC-P, WB, IF, E
Dilution	IHC-P~~1:50~100 WB~~1:1000 IF~~1:10~50
Synonyms	Endothelin-1, Preproendothelin-1, PPET1, Endothelin-1, ET-1, Big endothelin-1, EDN1
Images	EDN1 Antibody (C-term) (Cat. APR07642G)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EDN1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. EDN1 Antibody (C-term) (Cat. APR07642G) western blot analysis in ZR-75-1,U251,MDA-MB453,A549 cell line lysates (35ug/lane).This demonstrates the EDN1 antibody detected the EDN1 protein (arrow). Confocal immunofluorescent analysis of EDN1 Antibody (C-term)( APR07642G) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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