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SCUBE2 Antibody (Center N266)

AMM07730G Product Datasheet Protocol

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AMM07730G		100 μl / $495

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Leading Biology Inc.

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SCUBE2 Antibody (Center N266)

Brand	Leading Biology	Catalog Number	AMM07730G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality SCUBE2 antibody (Center N266).
Molecular Weight	109966 Da
Cellular Localization	Antigen Cellular Localization: Secreted.
Host	Rabbit
Species Reactivity	Human
Immunogen	251-280 aa
Target	This SCUBE2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 251-280 amino acids from the Central region of human SCUBE2.
Isotype	Rabbit Ig
Symbol	CEGP1
GeneID	57758
UniProt ID	Q9NQ36
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	IHC-P, WB, IF, E
Dilution	WB~~1:1000 IHC-P~~1:50~100 IF~~1:10~50
Synonyms	Signal peptide, CUB and EGF-like domain-containing protein 2, Protein CEGP1, SCUBE2, CEGP1
Images	Western blot analysis of lysates from Hela, ZR-75-1, HT-29 cell line (from left to right), using SCUBE2 Antibody (Center N266)( AMM07730G). AMM07730G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane. SCUBE2 Antibody (Center N266) (Cat. AMM07730G)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SCUBE2 Antibody (Center N266) for immunohistochemistry. Clinical relevance has not been evaluated. Confocal immunofluorescent analysis of SCUBE2 Antibody (Center N266)( AMM07730G) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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