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YOD1 Antibody (C-term)

Catalog # Availability Size / Price Inquiry
APR14430G 100 μl / $495

YOD1 Antibody (C-term)

Brand

Leading Biology

Catalog Number

APR14430G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality YOD1 antibody (C-term).

Molecular Weight

38322 Da

Host

Rabbit

Species Reactivity

Human

Immunogen

319-347 aa

Target

This YOD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 319-347 amino acids from the C-terminal region of human YOD1.

Isotype

Rabbit Ig

Symbol

DUBA8, HIN7, OTUD2

GeneID

UniProt ID

Function

Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Mediates deubiquitination of 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains. Also able to hydrolyze 'Lys-11'-linked ubiquitin chains. Cleaves both polyubiquitin and di-ubiquitin.

Summary

Deubiquitinating enzymes (DUBs; see MIM 603478) areproteases that specifically cleave ubiquitin (MIM 191339) linkages,negating the action of ubiquitin ligases. DUBA8 belongs to a DUBsubfamily characterized by an ovarian tumor (OTU) domain.[suppliedby OMIM].

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, IF, E

Dilution

WB~~1:1000 IHC-P~~1:50~100 IF~~1:10~50

Synonyms

Ubiquitin thioesterase OTU1, DUBA-8, HIV-1-induced protease 7, HIN-7, HsHIN7, OTU domain-containing protein 2, YOD1, DUBA8, HIN7, OTUD2

Images

YOD1 Antibody (C-term) (Cat. APR14430G) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the YOD1 antibody detected the YOD1 protein (arrow).

YOD1 Antibody (C-term) (Cat. APR14430G)immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of YOD1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Confocal immunofluorescent analysis of YOD1 Antibody (C-term)( APR14430G) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Specification

Quantity

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