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KRT25 Antibody (C-term)

Catalog # Availability Size / Price Inquiry
APR17101G 100 μl / $495

KRT25 Antibody (C-term)

Brand

Leading Biology

Catalog Number

APR17101G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality KRT25 antibody (C-term).

Molecular Weight

49318 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm {ECO:0000250|UniProtKB:Q8VCW2}.

Host

Rabbit

Species Reactivity

Human

Immunogen

380-408 aa

Target

This KRT25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-408 amino acids from the C-terminal region of human KRT25.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

Essential for the proper assembly of type I and type II keratin protein complexes and formation of keratin intermediate filaments in the inner root sheath (irs).

Summary

This gene encodes a member of the type I (acidic) keratinfamily, which belongs to the superfamily of intermediate filament(IF) proteins. Keratins are heteropolymeric structural proteinswhich form the intermediate filament. These filaments, along withactin microfilaments and microtubules, compose the cytoskeleton ofepithelial cells. The type I keratin genes are clustered in aregion of chromosome 17q12-q21.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, FC, E

Dilution

WB~~1:1000 FC~~1:10~50

Synonyms

Keratin, type I cytoskeletal 25, Cytokeratin-25, CK-25, Keratin-25, K25, Keratin-25A, K25A, Type I inner root sheath-specific keratin-K25irs1, KRT25 (HGNC:30839)

Images

KRT25 Antibody (C-term) (Cat. APR17101G) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the KRT25 antibody detected the KRT25 protein (arrow).

KRT25 Antibody (C-term) (Cat. APR17101G) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specification

Quantity

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