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EMX1 Antibody (C-term)

APR14109G Product Datasheet Protocol

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APR14109G		100 μl / $545

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Leading Biology Inc.

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EMX1 Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR14109G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality EMX1 antibody (C-term).
Molecular Weight	28039 Da
Cellular Localization	Antigen Cellular Localization: Nucleus {ECO:0000255\|PROSITE- ProRule:PRU00108, ECO:0000269\|PubMed:20887964}. Cytoplasm. Note=Might be shuttling between the nucleus and the cytoplasm
Host	Rabbit
Species Reactivity	Human, Mouse
Immunogen	228-257 aa
Target	This EMX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-257 amino acids from the C-terminal region of human EMX1.
Isotype	Rabbit Ig
GeneID	2016
UniProt ID	Q04741
Function	Transcription factor, which in cooperation with EMX2, acts to generate the boundary between the roof and archipallium in the developing brain. May function in combinations with OTX1/2 to specify cell fates in the developing central nervous system.
Summary	EMX1 is a transcription factor, which in cooperation with EMX2. It acts to generate the boundary between the roof and archipallium in the developing brain. It may function in combinations with OTX1/2 to specify cell fates in the developing central nervous system.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, FC, IF, E
Dilution	WB~~1:1000 FC~~1:10~50 IF~~1:10~50
Synonyms	Homeobox protein EMX1, Empty spiracles homolog 1, Empty spiracles-like protein 1, EMX1
Images	EMX1 Antibody (C-term) (Cat. APR14109G) western blot analysis in U251 and Neuro-2a cell line mouse cerebellum tissue lysates (35ug/lane).This demonstrates the EMX1 antibody detected the EMX1 protein (arrow). EMX1 Antibody (C-term) (Cat. APR14109G) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. Fluorescent confocal image of Hela cell stained with EMX1 Antibody (C-term)( APR14109G).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EMX1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min). EMX1 immunoreactivity is localized to Nucleus significantly.
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