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THBS1 Antibody (N-term)

APR07044G Product Datasheet Protocol

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APR07044G		100 μl / $545

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Leading Biology Inc.

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THBS1 Antibody (N-term)

Brand	Leading Biology	Catalog Number	APR07044G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality THBS1 antibody (N-term).
Molecular Weight	129383 Da
Cellular Localization	Antigen Cellular Localization: Endoplasmic reticulum. Sarcoplasmic reticulum
Host	Rabbit
Species Reactivity	Human
Immunogen	181-210 aa
Target	This THBS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 181-210 amino acids from the N-terminal region of human THBS1.
Isotype	Rabbit Ig
Symbol	TSP, TSP1
GeneID	7057
UniProt ID	P07996
Function	Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity). Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors (By similarity).
Summary	THBS1 is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis,and tumorigenesis.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, IF, FC, E
Dilution	WB~~1:1000 IHC-P~~1:10~50 IF~~1:10~50 FC~~1:10~50
Synonyms	Thrombospondin-1, THBS1, TSP, TSP1
Images	Western blot analysis of THBS1 Antibody (N-term) (Cat. APR07044G) in MDA-MB231 cell line lysates (35ug/lane). THBS1 (arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded human breast carcinoma reacted with THBS1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Confocal immunofluorescent analysis of THBS1 Antibody (N-term) ( APR07044G) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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