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Location: Home > Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > PRPS1/PRPS2/PRPS3 Antibody (N-term)

PRPS1/PRPS2/PRPS3 Antibody (N-term)

Catalog # Availability Size / Price Inquiry
APR10848G 100 μl / $545

PRPS1/PRPS2/PRPS3 Antibody (N-term)

Brand

Leading Biology

Catalog Number

APR10848G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PRPS1/PRPS2/PRPS3 antibody (N-term).

Molecular Weight

34834 Da

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

79-109 aa

Target

This PRPS1/PRPS2/PRPS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 79-109 amino acids from the N-terminal region of human PRPS1/PRPS2/PRPS3.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

Catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) that is essential for nucleotide synthesis.

Summary

Phosphoribosylpyrophosphate synthetase (PRPS; EC 2.7.6.1) catalyzes the phosphoribosylation of ribose 5-phosphate to 5-phosphoribosyl-1-pyrophosphate, which is necessary for the de novo and salvage pathways of purine, pyrimidine, and pyridine biosynthesis. By PCR of human lymphoblast mRNA using primers based on the cDNA sequence of rat PRS I (Prps1), Roessler et al. (1990) isolated a partial human PRPS1 cDNA. They used this partial cDNA to screen lymphoblast cDNA libraries and isolated additional cDNAs corresponding to the entire PRPS1 coding region. The deduced PRPS1 protein has 318 amino acids.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, E

Dilution

WB~~1:1000

Synonyms

Ribose-phosphate pyrophosphokinase 1, PPRibP, Phosphoribosyl pyrophosphate synthase I, PRS-I, PRPS1

Images

The anti-PRPS1/2/3 Pab (Cat. APR10848G) is used in Western blot to detect PRPS1/2/3 in mouse kidney tissue lysate (Lane 1) and Hela cell lysate (Lane 2).

Role of PPP during MSC transformation. (A) G6PD activity decreased during MSC transformation (& 8727;, P=0.0132; t test). Western blot confirms the down-regulation of G6PD and the up-regulation of PRPS at the late stages of transformation.

Specification

Quantity

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