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MCCC2 Antibody (Center)

Catalog # Availability Size / Price Inquiry
APR10893G 100 μl / $545

MCCC2 Antibody (Center)

Brand

Leading Biology

Catalog Number

APR10893G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality MCCC2 antibody (Center).

Molecular Weight

61333 Da

Cellular Localization

Antigen Cellular Localization: Mitochondrion matrix

Host

Rabbit

Species Reactivity

Human, Rat

Immunogen

163-189 aa

Target

This MCCC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-189 amino acids from the Central region of human MCCC2.

Isotype

Rabbit Ig

Symbol

MCCB

GeneID

UniProt ID

Function

Carboxyltransferase subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3- methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism.

Summary

MCCC2 is the small subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, FC, IHC-P, E

Dilution

WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50

Synonyms

Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial, MCCase subunit beta, 3-methylcrotonyl-CoA carboxylase 2, 3-methylcrotonyl-CoA carboxylase non-biotin-containing subunit, 3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta, MCCC2, MCCB

Images

MCCC2 Antibody (Center) (Cat. APR10893G) western blot analysis in NCI-H292 cell line and rat liver tissue lysates (35ug/lane).This demonstrates the MCCC2 antibody detected the MCCC2 protein (arrow).

Formalin-fixed and paraffin-embedded human hepatocarcinoma with MCCC2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of ATDC5 cells using MCCC2 Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specification

Quantity

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