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PRPF8 Antibody (C-term)

Catalog # Availability Size / Price Inquiry
APR12995G / $545

PRPF8 Antibody (C-term)

Brand

Leading Biology

Catalog Number

APR12995G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PRPF8 antibody (C-term).

Molecular Weight

273600 Da

Cellular Localization

Antigen Cellular Localization: Nucleus speckle.

Host

Rabbit

Species Reactivity

Human

Target

This PRPF8 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 2239-2273 amino acids from the C-terminal region of human PRPF8.

Isotype

Rabbit Ig

Symbol

PRPC8

GeneID

UniProt ID

Function

Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site.

Summary

Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, E

Dilution

WB~~1:2000

Synonyms

Pre-mRNA-processing-splicing factor 8, 220 kDa U5 snRNP-specific protein, PRP8 homolog, Splicing factor Prp8, p220, PRPF8, PRPC8

Images

Anti-PRPF8 Antibody (C-term)at 1:2000 dilution + 293T/17 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 274 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Specification

Quantity

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