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ATG4B Antibody (C-term)

AMM05346G Product Datasheet Protocol

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AMM05346G		100 μl / $545

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ATG4B Antibody (C-term)

Brand	Leading Biology	Catalog Number	AMM05346G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ATG4B antibody (C-term).
Molecular Weight	44294 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm.
Host	Rabbit
Species Reactivity	Human
Immunogen	358-390 aa
Target	This ATG4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 358-390 amino acids from the C-terminal region of human ATG4B.
Isotype	Rabbit Ig
Symbol	APG4B, AUTL1, KIAA0943
GeneID	23192
UniProt ID	Q9Y4P1
Function	Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C- terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Summary	Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4 is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	IF, WB, IHC-P, E
Dilution	IF~~1:100 WB~~1:1000 IHC-P~~1:50~100
Synonyms	Cysteine protease ATG4B, 3422-, AUT-like 1 cysteine endopeptidase, Autophagin-1, Autophagy-related cysteine endopeptidase 1, Autophagy-related protein 4 homolog B, hAPG4B, ATG4B, APG4B, AUTL1, KIAA0943
Images	Fluorescent image of U251 cells stained with ATG4B (C-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AMM05346G ATG4B (C-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ATG4B immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells. Western blot analysis of anti-hAPG4B-373 Pab (Cat. AMM05346G) in 293 cell line lysates transiently transfected with the ATG4B gene (2ug/lane). hAPG4B-373(arrow) was detected using the purified Pab. Western blot analysis of anti-APG4B Pab (Cat. AMM05346G) in Hela cell lysate. APG4B (arrow) was detected using the purified Pab.
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