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MBL2 Antibody (C-term)

APR06937G Product Datasheet Protocol

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APR06937G		100 μl / $495

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MBL2 Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR06937G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality MBL2 Antibody (C-term).
Molecular Weight	H=26 KDa
Cellular Localization	Antigen Cellular Localization: Secreted.
Host	Rabbit
Species Reactivity	Human
Immunogen	188-222 aa
Target	This MBL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-222 amino acids from the C-terminal region of human MBL2.
Symbol	COLEC1, MBL
GeneID	4153
UniProt ID	P11226
Function	Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.
Summary	This gene encodes the soluble mannose-binding lectin ormannose-binding protein found in serum. The protein encoded belongsto the collectin family and is an important element in the innateimmune system. The protein recognizes mannose andN-acetylglucosamine on many microorganisms, and is capable ofactivating the classical complement pathway. Deficiencies of thisgene have been associated with susceptibility to autoimmune andinfectious diseases.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IF, IHC-P
Dilution	IHC-P~~1:10~50 WB~~1:1000 IF~~1:10~50
Images	MBL2 Antibody (C-term) (Cat. APR06937G)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MBL2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. All lanes : Anti-MBL2 Antibody (C-term) at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: A549 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Confocal immunofluorescent analysis of MBL2 Antibody (C-term)( APR06937G) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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