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DAPK3 AntibodyBrand |
Leading Biology | Catalog Number |
AMM02977G |
Product Type |
Monoclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality DAPK3 Antibody.
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Molecular Weight |
52.5kDa
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Cellular Localization |
Antigen Cellular Localization:
Nucleus Cytoplasm. Note=Predominantly localizes to the cytoplasm but can shuttle between the nucleus and cytoplasm; cytoplasmic localization is promoted by phosphorylation at Thr-299 and involves Rho/Rock signaling. Isoform 2: Nucleus. Cytoplasm
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Host |
Mouse
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Species Reactivity |
Human
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Clone |
4H4C8
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Isotype |
IgG1
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Symbol |
ZIPK
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GeneID |
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UniProt ID |
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Function |
Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. Involved in the regulation of smooth muscle contraction. Regulates both type I (caspase- dependent) apoptotic and type II (caspase-independent) autophagic cell deaths signal, depending on the cellular setting. Involved in regulation of starvation-induced autophagy. Regulates myosin phosphorylation in both smooth muscle and non-muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A; the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton. Isoform 2 can phosphorylate myosin, PPP1R12A and MYL12B. Overexpression leads to condensation of actin stress fibers into thick bundles. Involved in actin filament focal adhesion dynamics. The function in both reorganization of actin cytoskeleton and focal adhesion dissolution is modulated by RhoD. Positively regulates canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Phosphorylates RPL13A on 'Ser-77' upon interferon-gamma activation which is causing RPL13A release from the ribosome, RPL13A association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Involved in regulation of cell cycle progression and cell proliferation. May be a tumor suppressor.
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Summary |
The protein encoded by this gene is an isozyme of very long-chain acyl-CoA synthetase (VLCS). It is capable of activating very long-chain fatty-acids containing 24- and 26-carbons. It is expressed in liver and associated with endoplasmic reticulum but not with peroxisomes. Its primary role is in fatty acid elongation or complex lipid synthesis rather than in degradation. This gene has a mouse ortholog. ; ;
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IHC, FC, E
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Dilution |
E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
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Images |
Black line: Control Antigen (100 ng);Purple line: Antigen(10ng);Blue line: Antigen (50 ng);Red line: Antigen (100 ng); Figure 1: Western blot analysis using DAPK3 mAb against human DAPK3 (AA: 28-161) recombinant protein. (Expected MW is 41.6 kDa) Figure 2: Western blot analysis using DAPK3 mAb against HEK293 (1) and DAPK3 (AA: 28-161)-hIgGFc transfected HEK293 (2) cell lysate. |
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Specification |
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Quantity |
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Select | Brand | Catalog No. | Product Name | Pack Size | Type | Field of Research | Specification | Quantity | Price(USD) | |
1 | Leading Biology | AMM07977G | SPRY1 / Sprouty 1 Antibody (clone 3H4) | 50 μg | Monoclonal Antibodies |
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$545.00 | Add Ask | ||
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$395.00 | Add Ask | ||
3 | Leading Biology | APG02467G | CCK4 / PTK7 Antibody (clone 4F9) | 50 μl | Monoclonal Antibodies |
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$495.00 | Add Ask | ||
4 | Leading Biology | AMM05365G | DC-SIGN Antibody [5D7] | 0.1 mg | Monoclonal Antibodies |
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$695.00 | Add Ask | ||
5 | Leading Biology | AMM01402G | Vimentin (Mesenchymal Cell Marker) Antibody - With BSA and Azide | 50 ug | Monoclonal Antibodies |
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$395.00 | Add Ask | ||
6 | Leading Biology | APR08280G | LTA4H / LTA4 Antibody (clone 9G8) | 50 μl | Monoclonal Antibodies |
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$495.00 | Add Ask |
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